How to inoculate in Laminar air flow chamber. Laminar flow (or streamline flow) occurs when a fluid flows in parallel layers, with no disruption between the layers. At low velocities the fluid tends to flow without lateral mixing, and adjacent layers slide past one another like playing cards. There are no cross currents perpendicular to the direction of flow, nor eddies or swirls of fluids. In laminar flow the motion of the particles of fluid is very orderly with all particles moving in straight lines parallel to the pipe walls.In fluid dynamics, laminar flow is a flow regime characterized by high momentum diffusion and low momentum convection.
A laminar flow cabinet or laminar flow closet or tissue culture hood is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive device. Air is drawn through a HEPA filter and blown in a very smooth, laminar flow towards the user. The cabinet is usually made of stainless steel with no gaps or joints where spores might collect.
Such hoods exist in both horizontal and vertical configurations, and there are many different types of cabinets with a variety of airflow patterns and acceptable uses.
Laminar flow cabinets may have a UV-C germicidal lamp to sterilize the shell and contents when not in use. (It is important to switch this light off during use, as it will quickly give any exposed skin sunburn and may cause cataracts.)
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