How to Make Mycelium Culture Slants. Culture slants are commonly used by mycologists to store fungal cultures long term. Using agar, we literally "slant" it in a test-tube, letting it cool. After that, we inoculate it with a culture (generally coming from a culture in a petri dish). The cultures can generally be stored for 2-3 years, but in some cases, as many as 5. When needed, the cultures can be accessed by scraping the slanted portion of the agar with a sterilized inoculation loop.
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